THE EFFECT OF ENCAPSULATED MULBERRY (Morus alba L.) LEAVES EXTRACT ON ARTERIAL BLOOD PRESSURE IN RATS

https://doi.org/10.22146/tradmedj.8372

Siti Aminah(1*), Suwaldi Suwaldi(2), Achmad Fudholi(3), Wahyono Wahyono(4)

(1) Faculty of Pharmacy, Universitas Gadjah Mada
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Pharmacy, Universitas Gadjah Mada
(4) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Rutin and quercetin are   flavonoid compounds containing in the extract of mulberry (Morus alba L.) leaves  have an influence on the cardiovascular system. This study aims to assessment of the blood pressure effect and cardiac puls after giving ethanolic extract of  mulberry leaves.  The extract was made using 95% ethanol by remaseration method. The content of rutin and quercetin in the extract were determined by using HPLC(High Performance Liquid Chromatography).  The formula of the encapsulation containing extract 0.25% w/v, chitosan 0.5mg/mL and TPP (sodium  tripolyphosphate) 1mg/mL. Characterization of extract encapsulated was done on the particle size, encapsulation efficiency and FTIR. Extract encapsulated was given to rats orally,  subsequently arterial blood pressure and heart rate were measured using Coda Non-Invasive Blood Pressure System. The results showed that mulberry extract contains rutin (0.879±0.138) mg/g  and  quercetin (4.427±0.065) mg/g. Ratio of chitosan-TPP with 0.5:1  forms particle size were   25.11-45.2 nm, encapsulation efficiency  (76.14±2.29)%. Mulberry leaves extract and encapsulated extract could decrease arterial blood pressure.  


Keywords


encapsulation, mulberry leaves extract, chitosan, blood pressure, heart rate

Full Text:

pdf


References

Ahmed,M., Akter, Mst.S., Lee, J-C., Eun, J-B., 2010, Encapsulation by spray drying of bioactive components, physicochemical and morphological properties from purple sweet potato, LWT – Food Science and Technology, 43, 1307 – 1312

Anonim, 1986, Sediaan Galenik, 4 – 16, Departemen Kesehatan Republik Indonesia, Jakarta

Aminah, S., Suwaldi, Fudholi, A., Wahyono, 2014, Enkapsulasi Ekstrak Daun Murbei (Morus alba L.) untuk Peningkatan Absorpsi In-Vitro Kandungan Rutin, JFI, 7,2, 105 - 110

Braunwald, E., 1982, Mechanism of Action of Calcium-Channel-Blocking Agents, N. Engl. J. Med. 307 (28), 1618 – 1627

Dalimartha, S., Abidin, M.A.C.M.M.., dan Winarno R., 1999, Atlas Tumbuhan Obat Indonesia, jilid 1 Cetakan 1, 90 – 96, Trubus Agriwidya, Jakarta

Duarte J, Perez-Vizcaino F, Zarzuelo A, Jimenez J, and Tamargo J., 1993, Vasodilator effects of quercetin in isolated rat vascular smooth muscle. Eur. J. Pharmacol., 239, 1–7

Höcht, C., Verniero, C.D., Opezzo, J.A.W., Bramuglia, G.F., Taira, C.A., 2006, Pharmacokinetic-pharmacodynamic (PK-PD) modeling of cardiovascular effects of metoprolol in spontaneously hypertensive rats: a microdialysis study, Naunyn-Schmiedeberg’s Arch Pharmacol, 373, 310 – 318

Edwards, R.L., Lyon, T., Litwin, S.E., Rabovsky, A. and Symons, J.D. , 2007, Quercetin Reduces Blood Pressure in Hypertensive Subjects, J.Nutr., 137, 2405 – 2411

Keele, C.A., Neil, E., Joels, N., 1982, Samson Wright’s Applied Physiology, 13th Ed., 65 – 152, Oxford University Press, New York

Lamina,B., Chemla, D., Richard, C., and Teboul, J-L., 2005, Clinical review : Interpretation of arterial pressure wave in shock states, Clinical Care, 9 (6), 601 – 606

Munin, A., and Edwards-Lévy, F., 2011, Encapsulation of Natural Polyphenolic Compounds ; a Review, Pharmaceutics, 3, 793 - 829

Nie, K., Tang, Z., Wu, X., Xu, X., Liang, Y., Li, H. and Rao, L., 2012, Optimization of total flavonoids extraction from mulberry leaf using an etanol-based solvent system, J. Med. Plants Res., 6 (12), 2373 – 2380

Perry, L.M., 1980, Medicinal Plants of East and South-east Asia, The MIT Press, London Paris, R., Plat,M., Giono-Barber, P., Linhard, J., Lawrence, A., 1977, Recherche Clinique et harmacologique Surlesfniler d’Anacardium occidumtale L., Soc. Med. Afr. Novu Lang, Franc, 22(3), 275 - 281

Rushmer, R.F., 1976, Structure and Function of the Cardiovascular System, 2nd Ed., W.B. Saunders Company, Philadelphia

Sinko, P.J., 2006, Martin’s Physical Pharmacy and Pharmaceutical Sciences, 5th Ed., 21- 58, Lippincott Williams&Wilkins, Philadelphia.

Snedecor, G.W. and Cochran, W.G., 1980, Statistical Methods, 7th Ed., 365 - 392, The Iowa State University Press, IOWA

Stoica, R., Somoghi, R. and Ion, R.M., 2013, Preparationof chitosan tripolyphosphate nanoparticles for the capsulation of polyphenols extracted from rose hips, Digest Journal of Nanomaterials and Biostructures, 8, 955-963

Stojanovic, R., Belscak-Cvitanovic, A., Manojlovic, V., Komes, D., Nedovic, V., Bugarski, B., 2012, Encapsulation of thyme (Thymus serpyllum L.) aqueous extract in calcium alginate beads, J. Sci. Food Agric., 92, 685–696

Wang, J., Wu, F.A., Zhao, H., Liu, L., Wu, Q.S., 2008, Isolation of flavonoids -from mulberry (Morus alba L.), African Journal of Biotechnology, 7(13) 2147-2155

Zhishen, J., Mengcheng, T., Jianming, W., 1999, The determination of flavonoid contenst in mulberry and their scavenging effects on superoxide radicals, Food Chemistry, 64, 555 – 559



DOI: https://doi.org/10.22146/tradmedj.8372

Article Metrics

Abstract views : 2384 | views : 4110

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2015 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada