ISOLATION AND IDENTIFICATION OF FREE RADICALS SCAVENGER FROM Daucus carota L LEAVES

https://doi.org/10.22146/tradmedj.8371

Sri Sudewi(1), Subagus Wahyuono(2), Puji Astuti(3*)

(1) Department of Pharmacy, University Sam Ratulangi
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


The presence of free radicals produced inside the cells is thought to cause a range of diseases such as degenerative diseases. Antioxidant properties of carrot root (Daucus carota L.) have been reported elsewhere to inhibit the formation of these free radicals, but the compounds responsible for antioxidant activity inside its leaves have not yet determined. In Indonesia, Daucus carota L. leaves are regarded as waste product after harvesting the root. This study was conducted to isolate and identify the compound having antioxidant activity from the leaves of D.carota. n-Hexane, ethyl acetate, and methanol extracts were examined for free radical scavenger activity by using DPPH method. The n-hexane extract showing free radical scavenger activity was solid-solvent partitioned, fractionated using vacuum liquid chromatography and tested for free radical scavenger activities. The active compound was isolated by using preparative thin layer chromatography and its purity was determined by TLC with three mobile phase systems and two dimensional TLC. Based on spectroscopy UV-Vis, IR as well as 1H-NMR (CDC13) data, the active compound was suggested to be xanthophyll derivate, lutein. The compound at concentration of 0.616, 1.025, and 2.05 ppm had antioxidant activities of 0.94 ± 0.05; 18.53 ± 0.15; and 49.07 ± 0.86, respectively.


Keywords


Leaves, D.carota, antioxidant, free radical

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References

Baranska, M, and Schulz, H., 2005, Spatial tissue distribution of polyacetylenes in carrot root, Analyst,130(6):855-9 Barra, A., Coroneo, V., Dessi, S., Cabras, P., Angioni, A., 2007, Characterization of the volatile constituents in the essential oil of Pistacia lentiscus L. from different origins and its antifungal and antioxidant activity, J Agric Food Chem., 55(17):7093-8 Bruneton, J., 1999, Pharmacognosy, Phytochemistry, Medicinal Plants, 2nd ed, translated by Halton, C.K., Lavoisier Publishing Inc, Intercept Ltd, Technique and Documentation, 769 Chew, B.P., Brown, C.M., Park, J.S., and Mixter, P.F., 2003, Dietary lutein inhibits mouse mammary tumor growth by regulating angiogenesis and apoptosis, Anticancer Res, 23, 3333-3339 Fang, Y.Z., Yang, S., and Wu, G., 2002, Free radicals, antioxidant and nutrition, Nutrition, 18, 872-879 Fessenden, R.J., dan Fessenden, J.S., 1995,Organic Chemistry, Willard Grant Press, Boston, (Indonesian), translated by Pudjaatmaka A.H., third ed., Erlangga, Jakarta Gowda, S.S.T., R. Dinesha, R. Harsha, and Srinivas, L., 2010, Free radical scavenging activity of lutein – isolated from Methi leaves (Trigonella foenum graecum), International Journal of Pharmacy and Pharmaceutical Sciences, 2, 113-117 Halliwell, B., and Gutteridge, J.M.C.,1985, Free radicals in biology and medicine, Oxford: Clorendon Press Halliwell, B., Gutteridge, J.M.C., and Cross, C.E., 1992, Free radicals, antioxidants and human disease: where are we now?, Journal of Laboratory and Clinical Medicine, 119, 598-620 Harborne, J.B., 1987. Phytochemical Methods. Chapman and Hall, London Hwang, S.W., Lee, J., Kwon, H.S., Lee, K.D., Nam, S.H., Park, K.H., and Yang, M.S., 2005, Comparison of tyronase inhibitory effect of the natural antioxidants from Cedrela sinensis, Agric. Chem. Biotechnol, 48 (3), 144-147 Kapoor, I.P., Singh, B., Singh, G., De Heluani, C.S., De Lampasona, M.P., Catalan, C.A., 2009, Chemistry and in vitro antioxidant activity of volatile oil and oleoresins of black pepper (Piper nigrum), J Agric Food Chem., 57(12):5358-64 Kim ,Y., Seo, J.H., Kim, H., 2011, β-Carotene and lutein inhibit hydrogen peroxide-induced activation of NF-κB and IL-8 expression in gastric epithelial AGS cells, J Nutr Sci Vitaminol (Tokyo), 57(3):216-23 Kogje, K.K., Jagdale, V.K., Dudhe, S.S., Phanikumar, G., and Badere, R.S., 2010, Antioxidant property and Phenolic Compounds of few Important Plants from Trans-Himalayan Regions of North India, Journal of Herbal Medicine and Toxicology, 4 (2) 145-151 Kour, H., 2006, Encyclopaedia of Plant Physiology, Vol 2, Anmol Publications Pvt.Ltd., New Delhi, India Kumar, S., Dhingra, A., and Daniell, H., 2004, Plastid-expressed betaine aldehyde dehydrogenase gene in carrot cultured cells, roots and leaves confers enhanced salt tolerance, Plant Physiology, 136, 2843-2854 Limsangouan, N., Takenaka, M., Sotome, I., Nanayama, K., Charunuch, C., and Isobe, S.,2010, Functional Properties of Cereal and Legume Based Extruded Snack Foods Fortified with By-Products from Herbs and Vegetables. Kasetsart J. (Nat. Sci.), 44, 271 – 279 Lopez-Lazaro, M., 2009, Distribution and biological activities of the flavonoid luteolin, Mini Reviews in Medicinal Chemistry, 9(1); 31-59 Mansou,r M.A., Ginawi, O.T., El-Hadiyah, T., El-Khatib, A.S., Al-Shabanah, O.A., Al-Sawaf, H.A., 2001, Effects of volatile oil constituents of Nigella sativa on carbon tetrachloride-induced hepatotoxicity in mice: evidence for antioxidant effects of thymoquinone, Res Commun Mol Pathol Pharmacol., 110(3-4):239-51 Masoko, P and Eloff, J.N., 2007, Screening of twenty-four South African Combretum and Six Terminalia Species (Combretaceae) for Antioxidant Activities, Afr. J. Trad. CAM, 4 (2): 231 - 239 Muralidharan, P., Balamurugan, G., and Kumar, P., 2008, Inotropic and cardioprotective effects of Daucus carota Linn. on isoproterenol-induced myocardial infarction. Bangladesh Journal of Pharmacology, 3, 74-79 Purup, S., Larsen, E., Christensen, L.P., 2009, Differential effects of falcarinol and related aliphatic C(17)-polyacetylenes on intestinal cell proliferation, J Agric Food Chem., 57(18):8290-6 Rohman A., Riyanto, S., Yuniarti, N., Saputra, W.R., Utami, R., and Mulatsih, W., 2010, Antioxidant activity, total phenolic, total flavonoid of extracts and fractions of red fruit (Pandanus conoidens Lam), Int.Food.Res.J.,17, 97-106 Roman, M., Dobrowolski, J.C., Baranska, M., Baranski, R., Spectroscopic studies on bioactive polyacetylenes and other plant components in wild carrot root., 2011, J Nat Prod., 74(8):1757-63 Ross, I.A., 2005, Medicinal Plants of the World volume 3 Chemical Constituents, Traditional and Modern Uses, Humana Press Inc, New Jersey Silverstein, R.M., Bassler, G.C., and Morril, T.C., 1991, Spectrometric Identification of Organic Compound, Fifth Edition, John Wiley and Sons, New York Smallwood, M., Worrall, D., Byass, L., Elias, L., Ashford, D., Doucet, C.J., Holt, C., Telford, J., Lillford, P., Bowles, D.J., 1999, Isolation and characterization of a novel antifreeze protein from carrot (Daucus carota)., Biochem J., 340 ( Pt 2):385-91 Sun, T., Simon, P.W., and Tanumihardjo, S.A., 2009, Antioxidant phytochemical and antioxidant capacity of biofortified carrots (Daucus carota L.) of various colors. Journal of Agriculture and Food Chemistry, 57, 4142–4147 Thomas, C. E., & Kalyanaraman, B.,1997, Oxygen radicals and the diseases process, The Netherlands: Hardwood Academic Publishers. Whaley-Connell, A., McCullough, P.A., Sowers, J.R., 2011, The role of oxidative stress in the metabolic syndrome, Rev Cardiovasc Med., 12(1):21-9 Yang, J., Guo, J., Yuan, J., 2008, In vitro antioxidant properties of rutin, LWT-Food Science and Technology, 41, 1060–106



DOI: https://doi.org/10.22146/tradmedj.8371

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