ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY TESTING OF PYRANON DERIVATED COMPOUND FROM ENDOPHYTIC FUNGI Penicillium sp OF KUNYIT PUTIH (Curcuma zedoaria (Berg) Roscoe)

https://doi.org/10.22146/tradmedj.8226

Muharni Muharni(1*), Fitrya Fitrya(2), Milanti oktaruliza(3), Elfita Elfita(4)

(1) Faculty of Mathematics and Science, Universitas Sriwijaya
(2) Faculty of Mathematics and Science, Universitas Sriwijaya
(3) Faculty of Mathematics and Science, Universitas Sriwijaya
(4) Faculty of Mathematics and Science, Universitas Sriwijaya
(*) Corresponding Author

Abstract


Exploration of bioactive compound were continuously done as more new diseases appearing, from infection, cancer, and other degenerative diseases. The invention of new antibiotic compound and antioxidant compound is very needed to solve this problem. Endophytic microbes are microbes which lives in plant’s culture and produce the active secondary metabolite. In previous research, 2 compounds from endofitic microbes Penicillium sp in kunyit putih had been successfully isolated and identified as di-(2-ethylhexyl)phthalate   and  pyranon derivated as 5 (4’-ethoxy-2’-hydroxy-5’-methyl-2’,3’-dihydrofuran-3’-il (hydroxy) methyl-4-isopropyl-3-methyl-2-pyran-2-on). The goal of this research is to test antibacterial and antioxidant activitiy from derivate pyranon, which is resulting from endofitic microbes Penicillium sp in kunyit putih (Curcuma zeodaria). The method used to test the antibacterial activity is dic diffusion, using E. Coli, S. Dysenteriae, S. Aureus, and B. Subtilis. with variation of concentrations 250, 500, 1000, dan 2000μg/mL, and to test the antioxidant activity used 1,1-diphenyl-2- picrylhydrazyl ( DPPH)  method with variation of concentrations 1000, 500, 250, 125, 62.5, 31.25, 15.625, and 7.8125 µg/mL. The research         shown that  the antibacterial activity is strongest at S. aureus with 11mm-blocked-zone at 250µg/mL  concentration. Pyranon derivate also has active antioxidant manner with IC5016.05µg/mL.


Keywords


antibacterial, antioxidant, pyranone, endophytic, Penicillium sp

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References

Departemen Kesehatan Republik Indonesia. 1988. Inventaris obat Indonesia. Jilid I. Badan penelitian dan pengembangan kesehatan. Jakarta

Hadioetomo, R. S. 1993. Mikrobiologi Dasar Dalam Praktek, Tehnik, dan Prosedur Dasar Laboratorium. Gramedia, Jakarta

Hundley, N. J. 2005. Struktur Elucidation of Bioactive Compounds Isolated from Endophytes of Alstonia Scholaris and Acmena Graveolens. Thesis, Department of Chemistry and Biochemistry. Brigham Young University

Hung, P. Q. and Annapurna, K .2004. Isolation and Characterization of Endophytic Bacterial in Soybean (Glycine sp.). Omonrice. 12, 92-101.

Lay, B.W. 1994. Analisis mikroba Laboratorium. PT. Raja Grafindo Persada, Jakarta

Minami, H., Hamaguchi, K., Kubo, M., and Fukuyama, Y. 1998. A benzophenone and a xanthone from Garcinia subelliptica. Phytochemistry. 49(6): 1783-1785

Muharni, Fitrya, Milanti Okta Ruliza, Dwi Anjar Susanti, and Elfita. 2014a. di-(2-ethylhexyl)phthalate and Pyranon Derivated from Endophytic fungi Penicillium sp the Leave of Kunyit Putih (Curcuma zedoaria (Berg) Roscoe). Indo.J.Chem. 14 (3), 290-296

Muharni, Fitrya, Milanti Okta Ruliza, Dwi Anjar Susanti, dan Elfita. 2014b. Aktivitas antioksidan di-(etilheksil)ftalat dari mikroba endofitik Penicillium sp Curcuma zedoaria (Berg.) Roscoe. Prosiding Semirata bidang MIPA BKS Barat. IPB. Bogor

Muharni, Fitrya, Milanti Okta Ruliza, Sri wahyuni, dan Elfita. 2014c. Aktivitas antibakteri senyawa di-(etilheksil)ftalat dari mikroba endofitik Penicillium sp pada tumbuhan kunyit putih Curcuma zedoaria (Berg.) Roscoe. Prosiding seminar MIPA net. UI. Jakarta

Pelctzar, J.M and Chan, E.C.S. 1988. Dasar-dasar Mikrobiologi. Edisi I. Terjemahan Ratna Siri dkk. UI-Press Jakarta

Prihatiningtias, W. 2005). Senyawa bioaktif Fungi Endofitik Akar kuning (Fibraurea chloroleuca Miers) sebagai senyawa antimikroba. Tesis. UGM. Yogyakarta

Radji, M. 2005. Peranan Bioteknologi dan Mikroba Endofit dalam Pengembangan Obat Herbal. Majalah Ilmu Kefarmasian. Vol 2. No.3 : 113 – 126

Selvi, A. T, Joseph, G. S., and Jayaprakasha,G. K. 2003. Inhibition of Growth and Aflatoxin Production in Aspergillus flavus by Garcinia indica Extract and Its Antioxidant Activity. Food Microbiology 20: 455-460

Tan, R.X., and Zou, W.X. 2001. Endophytes : A rich source of functional metabolites. Nat. Prod. Rep. 18 : 448-459



DOI: https://doi.org/10.22146/tradmedj.8226

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