Analysis of Curcumin Contents in Curcuma xanthorrhiza using FTIR Spectroscopy and HPLC-UV in Combination with Multivariate Calibration

https://doi.org/10.22146/mot.82220

Awal P. Kusumadewi(1), Rohmat Mujahid(2), Ronny Martien(3), Purwanto Purwanto(4), Anita A. Styawan(5), Irnawati Irnawati(6*), Abdul Rohman(7)

(1) *) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta *) Medicinal Plant and Traditional Medicine Research and Development Center, Tawangmangu, Central Java
(2) Research Center for Pharmaceutical Ingredients and Traditional Medicine of National Research and Innovation Agency (BRIN), Indonesia
(3) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
(4) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
(5) *) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta *) Faculty of Health and Technology, Universitas Muhammadiyah Klaten, Central Java
(6) Study Program of Pharmacy, Faculty of Pharmacy, Halu Oleo University, Kendari
(7) *) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta *) Centre of Excellence Institute for Halal Industry and Systems (PUI-PT IHIS), Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
(*) Corresponding Author

Abstract


Curcuma xanthorrhiza Roxb. or Java Turmeric with the local name of Temulawak is one of herbal medicines used in Indonesia. This plant is believed to prevent some degenerative diseases due to its active compounds, especially curcumin (CUR) contained in Java Turmeric. Therefore, analysis of active components including CUR is very urgent. This study highlighted the development of FTIR spectroscopy coupled with PLSR for the determination of CUR in Java Turmeric powders. The levels of CUR in Java Turmeric powders were determined using HPLC with UV detectors, and the obtained results were used as actual values to be predicted using FTIR spectroscopy-multivariate calibrations. The results revealed that the levels of CUR ranged from 0.6741 ± 0.0705% (g/100 samples) to 2.1062 ± 0.0095%. PLSR modeling for the relationship between the actual value of CUR as determined using HPLC and calculated values as predicted using FTIR spectroscopy provide the value of R2 of 0.9990 with RMSEC of 0.0028. The developed method offers reliable results providing a green analytical method due to the use of minimum solvent and reagent and does not involve extensive sample preparation.


Keywords


curcumin; PLS regression; HPLC; FTIR spectroscopy; chemometrics.

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DOI: https://doi.org/10.22146/mot.82220

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