ACTIVITIES TEST OF “JAMU GENDONG KUNYIT ASAM” (Curcuma domestica Val .; Tamarindus indica L.) AS AN ANTIDIABETIC IN STREPTOZOTOCIN-INDUCED RATS
Mohamad Andrie(1*), Wintari Taurina(2), Rizqa Ayunda(3)
(1) Faculty of Medicine, Universitas Tanjungpura
(2) Faculty of Medicine, Universitas Tanjungpura
(3) Faculty of Medicine, Universitas Tanjungpura
(*) Corresponding Author
Abstract
Diabetes mellitus is a metabolic disorder characterized by hyperglycemia. “Jamu gendong kunyit asam” (Curcuma domestica Val.; Tamarindus indica L.) is a traditional medicine that has antioxidant activity which can contribute to diabetic because of the phenolic content. This aim of this study is to determine the effect of “jamu gendong kunyit asam” on lowering blood glucose levels and repairing damage of the islet cells. 25 male wistar rats (Rattus norvegicus) were divided into five groups. Group I as a normal group without any treatment, group II, III, IV and V were induced with streptozotocin (7mg/ 200gBB) then treated with CMC 1%, glibenclamide (0.27mg / 200gBB), “jamu gendong kunyit asam” 1.90mL/ 200gBB and 3.80mL/ 200gBB. The treatment was done for 28 days. Glucometer was used to measure the gloucose blood level on day 4, 8, 12, 16, 20, 24 and 28. The damages on the islet cells were examined under the microscope on the histology sample of pancreas prepared with Hematoxyllin eosin stain. Datas were analyzed statistically with One Way Anova test, T-Test, and the Kruskal-Wallis test using SPSS 17.0 for Windows. Results showed there is a significant difference (p> 0.05) in blood glucose levels and damage to the islets of Langerhans of the pancreas between the glibenclamide group and treatment of “jamu gendong kunyit asam”. From the research it can be concluded that the “jamu gendong kunyit asam” have antidiabetic activity which is showed by a decrease in blood glucose levels and an improvement of pancreatic islets of Langerhans in streptozotocin-induced diabetic rats with an effective dose of 1.90mL/ 200gBB.
Keywords
Full Text:
pdf (Bahasa Indonesia)References
Atangwho, I. J., Ebong, P. E., Egbung, G. E., dan Obi, A. U., 2010, Extract of Vernonia amygdalina Del. (African Bitter Leaf) Can Reverse Pancreatic Cellular Lesion after Alloxan Damage in the Rat, Australian Journal of Basic and Applied Sciences, 4 (5): 711-716
Barbosa, D. S., 2007, Green Tea Polyphenolic Compounds and Human Health, Journal of Consumer Protection and Food Safety, 2: 407-413
Bhutkar, M. A. dan Bhise, S. B., 2011, Anti-Oxidative Effect of Tamarindus indica in Alloxan Induced Diabetic Rats, International Journal of Research and Biomedical Science, 2 (3): 1006-1009
Botutihe, 2010, Efek Ekstrak Rumput Laut Coklat (Sargasum duplicatum Bory) Terhadap Profil Radikal Bebas dan Protein Kinase C Paru Tikus (Rattus norvegicus) yang Dipapar Benzo[A]piren, Tesis, Malang: Universitas Brawijaya
Bunting, K., Wang, J. K, dan Shannan, M. F., 2006, Control of Interleukin-2-gene Transcription: A Paradigm for Inducible, Tissue Speciic Gene Expression, Elsevier Academic Press, 74: 105-145
Dipiro, J. T., 2005, Pharmacotherapy Handbook, The Mc. Graw Hill Company: USA
El-Masry, A. A., 2012, Potential Therapeutic Effect of Curcuma longa on Streptozotocin Induced Diabetic Rats, Glo. Adv. Res. J. Med. Med. Sci, 1 (4): 091-098
Endang, S., 2000, Membuat Jamu Beras Kencur, Kanisius Media: Yogyakarta
Evans, J. L., Goldfine, I. D., Maddux, B. A., dan Grodsky, G.M., 2003, Are Oxidative Stress-Activated Signaling Pathways Mediators of Insulin Resistance and β Cells Dysfunction. Diabetes Journal. 52 (1): 1-18
Hones, J., Muller, P., dan Surrige, N., 2008, The Technology Behind Glucose Meters: Test Strips, Diabetes Technol Ther, 10: 10-26
Koneri, R.B., Samaddar, S., dan Ramaiah, C.T., 2014, Antidiabetic activity of a triterpenoid saponin isolated from Momordica cymbalaria Fenzl, Indian Journal Exp Biol, 52 (1): 46-52
Meliani, N., Dib, M. A., Allali, H., dan Tabti, B., 2011, Hypoglycaemic effect of Berberis vulgaris L. in normal and streptozotocin-induced diabetic rats, Asian Pacific Journal of Tropical Biomedicine, 1 (6): 467-471
Murray, R. K., Granner, D. K., Mayes, P. A., dan Rodwell, V. W., 1999, Biokimia Herper, Penerjemah: Hartono, A., EGC: Jakarta
Padayatty, S. J., Katz, A., Wang, Y., Eck, P., Kwon, O., dan Lee, J. H., 2003, Vitamin C as an Antioxidant: Evaluation of its Role in Disease Prevention, Journal of American College of Nutrition, 22: 18-35
Sabu, M. C., Smitha, K., dan Ramadasan, K., 2002, Anti-diabetic Activity of Green Tea Polyphenols and Their Role In Reducing Oxidative Stress In Experimental Diabetes, Journal Ethnopharmacol, 83: 109-116
Sandhar, H.K., Kumar, B., Prashes, P., Tiwari, P., Salhan, M., dan Sharma, P., 2011, A Review Of Phytochemistry and Pharmacology Of Flavonoids, Internationale Pharmaceutica Scienca, 1 (1): 25-41
Suarsana, I. N., Priosoeryanto, B. P., Bintang, M., dan Wresdiyati, T., 2010, Profil Glukosa Darah dan Ultrastruktur Sel Beta Pankreas Tikus yang Diinduksi Senyawa Aloksan, JITV, 2 (15): 118-123
Sutarno, H. S. A., 2000, Potensi dan Cara Pemanfaatan Bahan Tanaman Obat, Prosea Indonesia: Bogor
Szkuldelski, T., 2001, The Mechanism of Alloxan and Streptozotocin Action in Cells of The Rat Pancreas, Physiol Res, 50: 536-546
Tapas, A. R., Sakarkar, D. M., dan Kakde, R. B., 2008, Flavonoid as Natraceuticals: A Review, Tropical Journal of Pharmaceutical Research, 7 (3): 1089-1099
Tjokroprawiro, A., 1996, Diabetes Mellitus, Klasifikasi, Diagnosis, dan Terapi Edisi ke 3, Gramedia Pustaka: Jakarta.
Yusup, N., 2001, Kajian Aktivitas Antioksidan Minuman Tradisional Hasil Olahan Industri, Skripsi, Bogor: Institut Pertanian Bogor
DOI: https://doi.org/10.22146/tradmedj.8147
Article Metrics
Abstract views : 5892 | views : 15848Refbacks
- There are currently no refbacks.
Copyright (c) 2015 Majalah Obat Tradisional
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Faculty of Pharmacy
Universitas Gadjah Mada