ANTIBACTERIAL ACTIVITY TESTING OF N-HEXANE FRACTION OF RED DRAGON (Hylocereus polyrhizus Britton & Rose) FRUIT PEEL ON Staphylococcus aureus ATCC 25923

https://doi.org/10.22146/tradmedj.8146

Sri Amalia(1*), Sri Wahdaningsih(2), Eka Kartika Untari(3)

(1) Faculty of Medicine, Tanjungpura University
(2) Faculty of Medicine, Tanjungpura University
(3) Faculty of Medicine, Tanjungpura University
(*) Corresponding Author

Abstract


Inappropiate antibiotic use was one of the main reason that caused pathogenic microorganism resistance, leads to ineffective treatment. One of those resistance bacteria is Staphylococcus aureus. The research for discovering new antibiotics can help with this issue. The peel of red dragon fruit (Hylocereus polyrhizus) is one of the plants that has antibacterial compounds. The aim of this research is to prove and determine the clear zone value of n-hexane fraction of red dragon fruit’s peel againts Staphylococcus aureus ATCC 2593. The peel was maserated with chloroform, then fractinated by n-hexane. Futhermore, the antibacterial properties of the fraction was tested using Disc Diffusion (Kirby-Bauer) methode with 20 and 40 mg/mL concentrations. Ampicillin used as control positive, and Dymetil sulfoxida (DMSO) used as control negative . Based on the phytochemical screening of the fraction, the n-hexane fraction of the dragon fruit’s peel contain terpenoid and alkaloid. Clear zones produced by 40 mg/mL is 12.80±1,69 mm and for 20 mg/mL is 11.17±1,11 mm.

Keywords


Antibacterial, n-Hexane fraction, Red dragon fruit’s peel, Staphylococcus aureus ATCC 2593



References

Ahmad, I dan Aqil, F. 2007. In vitro efficacy of bioactive extracts of 15 medicinal plants against ESβL-producing multidrug-resistant enteric bacteria. Microbiological Research.162(3):264–275

Barbour, E.K., Al Sharif, M. Dan Sagherian, V.K. 2009. Screening of selected indigenous plants of Lebanon for antimicrobial activity. Journal of Ethnopharmacology.93(1):1–7

Cowan, M. 1999. Plant Product as Antimicrobial Agent. Clin Microbiol Rev.12(4): 564-582

Departemen Kesehatan RI. 1988. Inventaris Obat Indonesia Jilid I. Badan Penelitian dan Pengembangan Kesehatan, Jakarta

Guarrera, P.M. 2005. Traditional phytotherapy in Central Italy (Marche, Abruzzo, and Latium). Fitoterapia.76(1):1–25

Indian Council of Medical Research. 2009. Detection of Antimicrobial Resistance in Common Gram Negative and Gram Positive Bacteria Encountered in Infectious deseases- An Update. ICMR Bulletin.39:1-3

Kaper, JB., Nataro, J.P dan Mobley, H.L. 2004. Pathogenic Staphylococcus aureus. Nat. Rev. Microbiol. 2:123-140

Khalili, M.A., Norhayati, A.H., Rokiah, MY., Asmah, R., Muskinah, M.S. dan Manaf, A.A. Hypocholesterolemic Effect of Red Pitaya (Hylocereus sp.) on Hypercholesterolemia Induced Rats. Int. Food Res. J. 16:431-440

Kristianto, D. 2008. Buah Naga Pembudidayaan di Pot dan di Kebun. Penebar Swadaya, Jakarta

Lamothe, R.G., Mitchell, G., Gattuso, M., Diarra, M.S. dan Malouin, F. 2009. Plant Antimicrobial Agents and Their Effects on Plant and Human Pathogens. International Journal of Molecular Sciences.10:3400-3419

Latif, A.Z., Haque, M. dan Shanmugasundaram, C. 2012. Clinical study of preventive potentials of consumption of Buah naga [Cactaceae] against paracetamol– induced hepatotoxicity as well as the other associated biological effects. Asian J. Res. in Pharm. Sci. 2(1):16-23

Luo, H., Cai, Y., Peng, Z., Liu, T dan Yang, S. 2014. Chemical Composition and In Vitro Evaluation of The Cytotoxic and Antioxidant Activities of Supercritical Carbon Dioxide Extracts of Pitaya (Dragon Fruit) Peel. Chem. Central J.8(1):2-7

Melliawati, Ruth. 2009. Esherichia coli dalam Kehidupan Manusia. BioTrend.4(1)

Nurmahani, M.M., Osman, A., Abdul Hamid, A., Mohamad Ghazali, F. dan Pak Dek, M.S. Short Communication Antibacterial Property of Hylocereus polyrhizus and Hylocereus undatus Peel Extracts. Int. Food Res. J. 2012;19(1):77-84

Phebe, D., Chew, M. K., Suraini, A. A., Lai, O. M. dan Janna, O. A. 2009. Red-fleshed pitaya (Hylocereus polyrhizus) fruit colour and betacyanin content depend on maturity. Int. Food Res. J.16:233-242

Tahany, M.A.A., Hegazy, A.K., Sayed, A.M., Kabiel, H.F., El-Afry, T. dan El-Komy, S.M. 2010. Study on combined antimicrobial activity of some biologically active constituents from wild Moringa peregrina Forssk. J. Yeast Fungal Res. 1(1):15-24

Uddin, G., Feroz, S., Ali, J. dan Rauf, A. 2014. Antioxidant, antimicrobial activity and phytochemical investigation of Pterospermum acerifolium (Leaf petiole). J. Agricul. Res. 3(3):058-062

Warisno, Dahana K. 2010. Cara Pintar Bertanaman Buah Naga di Kebun, Pekarangan dan dalam Pot. PT. Gramedia Pustaka Utama, Jakarta

Wu, L., Hsu, H., Chen, Y., Chiu, C., Lin, Y. dan Ho, J.A. 2006. Antioxidant and antiproliferative activities of red pitaya. J. Food Chem.95(2):319-327



DOI: https://doi.org/10.22146/tradmedj.8146

Article Metrics

Abstract views : 5579 | views : 15877

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2015 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada