AWAR–AWAR (Ficus septica Burm. f.) LEAVES ETHANOLIC EXTRACT INDUCED APOPTOSIS OF MCF-7 CELLS BY DOWNREGULATION OF Bcl-2

https://doi.org/10.22146/tradmedj.8133

Dewi Arum Sekti(1), Muhammad Fithrul Mubarok(2), Inna Armandani(3), Sendy Junedy(4), Edy Meiyanto(5*)

(1) Faculty of Pharmacy, Universitas Gadjah Mada
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Pharmacy, Universitas Gadjah Mada
(4) Faculty of Pharmacy, Universitas Gadjah Mada
(5) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Alkaloid fenantroindolisidin showed cytotoxic activity against several cancer cells. Ethanolic leaf extract of Awar-awar proven to increase the cytotoxic activity of chemotherapy agent doxorubicin in MCF-7 cells. This study aims to determine the effect of Ethanolic leaf extract of Awar-awar in apoptosis induction of breast cancer cells MCF-7.Awar-awar leaf powder was extracted by maceration using 70% ethanol and then concentrated to obtain concentrated of Awar-awar. Apoptosis induction was examined by double staining method and the expression of Bcl-2 protein was observed by  immunohistochemistry method. Single treatment of Awar-awar leaf extract  showed the ability of extracts to induce apoptosis and downregulated the expression of Bcl-2 protein. Ethanolic leaf extract of Awar-awar is potential as co-chemotherapeutic agent.

Keywords


Awar-awar (F. septica Burm. f.), MCF-7 cells, apoptosis, Bcl-2



References

Damu, Amooru G., Kuo, Ping-Chung, Shi, Lian Shi, Li, Chia-Ying, Kuoh, Chang-Sheng, Wu, Pei-Lin, Wu, and Tian-Shung, 2005, Phenanthroindolizidine Alkaloids from The Stems of Ficus septica, J. Nat. Prod., 68, 1071-1075

King, R.J.B., 2000, Cancer Biologi, 2nd edition, 5, Pearson Education Limited, London

Liang, Y., Yan, C., and Schor, N.F., 2001, Apoptosis in The Absence of Caspase 3, Oncogene, 20, 6570-6578

Manikdanan P., Vidjaya Letchoumy P., Prathiba D., and Nagini S., 2007, Proliferation, angiogenesis dan apoptosis-associated proteins are molecular targets for chemoprevention of MNNG-induced gastric carcinogenesis by ethanolic Ocimum sanctum leaf extract, Singapore Med J, 48 (7), 645–651

McGahon, A. J., Martin, S. J., Bissonnette, R. P., Mahboubi, M., Shi, Y., Mogil, R. J., Nishioka, W. K., and Green, D. R., 1995, The End of the (Cell) Line: Methods for the Study of Apoptosis in Vitro, Cell Death, Academic Press, San Diego

Mubarok M.F., Sekti Dewi A., and Ainun W., 2008, Peningkatan Aktivitas Sitotoksik Doxorubicin terhadap Sel MCF-7 menggunakan Ekstrak Etanolik Daun Awar-awar (Ficus septic Burm. f.), Prosiding KONGRES ILMIAH XVI ISFI 2008. ISBN : 978-979-95107-6-2. Penerbit : Ikatan Sarjana Farmasi Indonesia

Nurcahya, B. M., 2007, Efek Antiproliferatif Ekstrak Etanolik Daun Awar-Awar (Ficus septica Burm. f.) terhadap Sel Kanker Payudara T47D, Skripsi, Fakultas Farmasi UGM, Yogyakarta

Padanilam, B.J., 2003, Cell Death Induced by Acute Renal Injury: A Perspective on the Contributions of Apoptosis dan Necrosis, Am J Physiol Renal Physiol, 284, F608–F627

Ricci, M.S. and Zhong, W.X., 2006, Chemoterapeutic Appoaches for Targeting Cell Death Pathways, The Oncologist, 11, 342-357

Simstein, R., Burow, M., Parker, A., Weldon, C., and Beckman, B., 2003, Apoptosis, Chemoresistance and Breast Cancer : Insights from the MCF-7 Cell Model System, Exp.Biol.Med., 228, 995-1003

Staerk, D, Lykkeberg,A.K, Christensen, J, Budnik,B.A., Abe, F. and Jaroszewski,J.W., 2002, In Vitro Cytotoxic Activity of Phenanthroindolizidine Alkaloids from Cynanchum vincetoxicum dan Tylophora tanakae against Drug-Sensitive dan Multidrug Resistant Cancer Cells, J. Nat. Prood, 65, 1299-1302



DOI: https://doi.org/10.22146/tradmedj.8133

Article Metrics

Abstract views : 2710 | views : 9499

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2010 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada