IDENTIFICATION OF COMPOUND ANTIFUNGI OF Sandoricum koetjape. Stem AND ACTIVITY TO Candida albicans

https://doi.org/10.22146/tradmedj.8055

Warsinah Warsinah(1*), Eka Kusumawati(2), Sunarto Sunarto(3)

(1) Faculty of Medicine and Health Science, University of Jenderal Soedirman
(2) Faculty of Medicine and Health Science, University of Jenderal Soedirman
(3) Faculty of Medicine and Health Science, University of Jenderal Soedirman
(*) Corresponding Author

Abstract


Kecapi (Sandoricum koetjape (Burm.f.) Merr) was one of the traditional medicine from  family Meliaceae and had compounds such as flavonoids, saponins and polyphenols that can be used as antifungal. The objective of this research to know compounds contained from S. koetjape bark that has potential as an antifungal toward C. albicans.Research carried out two stages using  extraction by maceration with methanol and fractionation by column chromatography method used the solvent n-hexane, chloroform, ethyl acetate and methanol. The next step was examined its activity toward C. albicans and identification of fraction active compounds with the Gas Chromatograpy-Mass Spectrometry (GC-MS). The results of this research showed that methanol extract from S. koetjape bark had antifungal activity againstC. albicans (39,654%). Next, the methanol extract was fractionated and fractionation results were obtained six fractions with the greatest antifungal activity of fraction III, followed by fraction V (39.66% b/v), fraction IV (39.02% b/v), fraction II (37.91% b/v), fraction VI (35.48 % b/v) and fraction I (33.72% b/v). Identification of compounds with GC-MS in fraction III and compounds contained have potential as an antifungal are α-gurjunene, trans-caryophyllene, aromadendrene, α-humulene, β-caryophyllene, δ-Cadinene, alloaromadendrene, octadecanoic acid (as. stearat), hexadecanoic acid metil ester (metil palmitate), hexadecanoic acid (as. palmitate), 9-octadecenoic acid metil ester (metil elaidate), 9-octadecenoic acid (as. oleat).

Keywords


Candidaalbicans, Fractionation, Identification, Sandoricumkoetjape



References

Ali, N.A., Martina W., N. Arnold, U. Lindequist, L. Wessjohan, 2008, Essential Oil Composition from Oleogum Resin of Soqotraen Commiphora kua, Rec. Nat. Prod. 2 (3) : 70-75, www.acgpubs.org/RNP

Asghar, S., Rehman, M.I. Choudahry, and Rahman, 2011, Gas chromatography-mass spectrometry (GC-MS) analysis of petroleum ether extract (oil) and bio-assays of crude extract of Iris germanica, International Journal of Genetics and Molecular Biology3 (7):95-100, http: //www. academicjournals .org /ijgmb

Cowan, M.M., 1999, Plant Products as Antimikrobial Agents, Clinical Microbiology Review, 12 (4) : 564 – 582, http: //www. heart-intl.net/HEART/120104/Plant Productsas Antimicrobi.pdf

Djumidi, H. 1997, Invertaris Tanaman Obat Indonesia. Jilid IV, Badan Penelitian dan Pengembangan Kesehatan, Departemen Kesehatan dan kesejahteraan Sosial RI, Jakarta

Guo, L., Jin-zong W., Tin H., Tong C.,and Khalid R., 2008, Chemical Composition, Antifungal and Antitumor Properties of Ether Extracts of Scapania verrucosa Heeg. and its Endophytic Fungus Chaetomium fusiforme, Molecules, 13 : 2114-2125, DOI: 10.3390/molecules 13092114, www.mdpi.org/molecules

Hustani, M., 2009, Uji Aktivitas Antibakteri Ekstrak Etanol Daun Salam (Syzygium polyanthum [Wigh] Walp.) Terhadap Bakteri Penyebab Diare, Skripsi, Fakultas Kedokteran dan Ilmu-Ilmu Kesehatan, Purwokerto, Tidak dipublikasi

Kosenda, L., 2009, Penampisan Senyawa Bioaktif dari Daun Jambu Biji (Psidium guajava L.) sebagai Anticandidiasis dan Profil Kromatografi Lapis Tipisnya,Skripsi, Fakultas Kedokteran dan Ilmu-Ilmu Kesehatan, Purwokerto, Tidak dipublikasi

Lenny, S., 2006, Senyawa Terpenoid dan Steroid, http://library.usu.id/download/fmipa/06003488.pdf

Noviyanti, L. 2010, Modifikasi Teknik Kromatografi Kolom untuk Pemisahan Trigliserida dari Ekstrak Buah Merah(Pandanus conoideus Lamk.), Skripsi, Universitas Sebelas Maret, Surakarta, http://eprints.uns.ac.id/389/1/168090609201010021.pdf

Padmini, E.A., Valarmathi, A., and Rani, M.U., 2010, Comparative Analysis of Chemical Composition and Antibacterial Activities of Mentha spicata and Camellia sinennsis. Asian J. Exp. Biol. Sci, 1 (4) : 772 - 781, http://www.ajebs.com/vol-4/9a.pdf

Panda, K., S.S. Brahma and K. Dutta, S., 2010, Selective Antifungal Action of Crude Extracts of Cassia fistula L.: A Preltminary Study on Candida and Aspergillus spesies, Malaysian Journal of Microbiology, 6(1):62-68, http://web.usm.my/mjm/issues/vol6no1/research9.pdf

Riswiyanti, A., 2002, Uji Aktivitas Antimikroba dan Profile KLT Fraksi Metanol Kulit Batang Kecapi (Sandoricum koetjape (Burm.f.) Merr.) TerhadapStaphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Shigella disenteriae dan Candida albicansi, Skripsi, Universitas Gadjah Mada, Yogyakarta, Tidak dipublikasi

Rochani, N., 2009, UjiAktivitas Antijamur Ekstrak Daun Binahong ( Anrederacordifolia (Tenore). Steen) Terhadap Candida albicans serta Skrining Fitokimianya, Skripsi, Universitas Muhamadiyah Surakarta, Surakarta, ,http://etd.eprints.ums.ac.id /5267/1/K100050305.pdf

Setyaningsih, I., Desniar, Tresna S., 2005, Konsentrasi Hambatan Minimum Ekstrak Chlorella sp.Terhadap Bakteri dan Kapang, Buletin Teknologi Hasil Perikanan, VIIINo. 1: 25-34, http://202.124.205.107/files/ IrianiSetyaningsih_KonsentrasiHambatanMinimum.pdf

Soemiati, Atiek dan Berna Elya, 2002. Uji Pendahuluan Efek Antijamur Infus Daun Sirih (Piper betle L.), Kulit Buah Delima (Punica granatum L.) dan Rimpang Kunyit (Curcuma domestica Val.) Terhadap Jamur Candida albicans, Makara,Seri Sains, 6 No. 3, http://journal.ui.ac.id/upload/artikel/Uji%20Pendahuluan%20Efek_Atiek%20S.%20Berna.pdf

Sundari, D. dan M.W. Winarno, 1996, Efek Farmakologi dan Fitokimia Komponen Penyusun Jamu Keputihan, http://www. kalbe.co.id/files/cdk/files/07EfekFarmakologiFitokimiaKomponen108.pdf/07EfekFarmakologiFitokimiaKomponen108.html

Swantara, Dira dan Yenni, 2009, Identifikasi Senyawa Antibakteri Pada Daun Kecapi(Sandoricum koetjape (Burm.f.)), Jurnal Kimia(3) : 61-68, http://ejournal. unud.ac.id/abstrak/j%20kim%20vol%203%20no%202%20-1.pdf



DOI: https://doi.org/10.22146/tradmedj.8055

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