FREE RADICAL SCAVENGING ACTIVITY OF (Alsophila glauca J. Sm)

https://doi.org/10.22146/tradmedj.8053

Sri Wahdaningsih(1*), Erna Prawita Setyowati(2), Subagus Wahyuono(3)

(1) Faculty of Medicine and Health Science, University of Tanjungpura
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Free radical induced oxidative stress that influences the occurrence of various degenerative diseases such as cancer, coronary heart disease and premature aging. In the case that body's antioxidant defense system does not have excessive antioxidants, additional natural antioxidant via food or other nutrients intake is needed. Stems of ferns are known to contain phenolic compounds that are known to have antioxidant activity. A study has been carried out to determine antioxidant potential of stems of ferns of western Borneo origin compared to quercetin as positive control. Initially, material was macerated gradually with wasbenzen followed by methanol. The extract obtained was filtered and evaporated to dryness to give dried wasbenzen extract and methanol extract respectively. Antioxidant properties of extracts were performed by DPPH on TLC. Active (wasbenzen) extract was then partitioned with 80% methanol to give soluble and insoluble fractions. Active (insoluble) fraction was further fractionated by vacuum liquid chromatography using a gradient mobile phase with increasing polarity (wasbezen: chloroform). Fractions obtained were tested antioxidant activity, and isolation was done by preparative TLC. The purity of isolated antioxidant compound was confirmed by TLC, and antioxidant potential was measured spectroscopically with DPPH method. This compound displayed antioxidant activity, but its value was lower than that of quercetin used as a positive control (IC50 178.4 v / s 2.17 microg / mL)

Keywords


fern stem, antioxidant, DPPH (2,2-Difenil-1 pikrilhidrazin)



References

Blouis, M. S., 1958, Antioxidant Determinations By The Use Of a Stable Free Radical, Nature, 1199-1200

Demirezer, L.O., Kruuzum-Uz, A., Bergere, I., Schiewe, H.J., & Zeeck, A., 2001, The Structures of Antioxidant and Cytotoxic Agents from Natural Source : Antraquinones and Tannin from Roots of Rumex patientia, Phytochemistry, 58: 1213-1217

Halliwel, B. & Gutteridge, JMC., 1999, Free Radical in Biology and Medicine, 3th ed., pp.1-231, 353-425, Oxford University Press, Inc., New York

Hanani, E, 2005, Hanani, E, A. Mun’im, R. Sekarini, Identifikasi Senyawa Antioksidan Dalam Spons Callyspongia SP Dari Kepulauan Seribu, Majalah Ilmu Kefarmasian, Vol II, No 3 (2005). Page 127-133

Kwon, Y.S., & Kim, C.M., 2003, Antioxidant Constituent from the Stem of Sorghum bicolor, Arch. Pharm. Res., 26 (7) : 535-539

Maisuthisakul, P., & Pasuk, S., 2007, Antioxidant Properties and Phenolic Phytochemicals From Various Cultivars of Thai Mango Seed Kernels, laporan penelitian, University of Thai Chamber of Comerce, Bangkok

Permana, D.,N. Hj. Lajis, Faridah Abas, A. Ghafar othman, Rohaya Ahmad, Mariko Kitajama, Hiromitsu Takayama, Nario Aimi, Cl, 2003, Antioksidative Constituents Of Hedotis Diffusa Wild “., Natural Product Sciences, 9(1), 7-9

Rohdiana, D., 2001, Radical Scavengers Polyphenol, MFI., 12 (1) : 53-58



DOI: https://doi.org/10.22146/tradmedj.8053

Article Metrics

Abstract views : 3159 | views : 156660

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2015 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada