ANTIOXIDANT ACTIVITY ANALYSIS OF Cynara scolimus L., Artemisia china L., Borreria repensDC.,Polygala paniculata L. FROM TAMAN NASIONAL GUNUNG MERAPI USING DPPH (2,2-DIFENIL-1-PIKRILHIDRAZIL) RADICAL SCAVENGING ANALYSIS

https://doi.org/10.22146/tradmedj.8011

Paulus Eko Murwanto(1), Djoko Santosa(2*)

(1) Faculty of Pharmacy, Universitas Gadjah Mada
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Taman Nasional Gunung Merapi (TNGM) has various biodiversities which need to be explored. This study aims to analyze antioxidant activity four plants from Asteraceae, Rubiaceae and Polygalaceae which were collected from TNGM. Antioxidant activity is analyzed by DPPH radical capturing method using spectrophotometer. Sampel is extracted gradually by wasbenzen and methanol. DPPH radical capturing analysis shows that IC50 score of Cynara scolimus L. wasbenzen extract is 496,16g/mL; Artemisia china L. 407,86g/mL; Borreria repens DC. 984,04 g/mL and Polygala paniculata L. 633,34 g/mL. The IC50 score of methanol extract Cynara scolimus L. is 334,94g/mL; Artemisia china L. 199,99g/mL; Borreria repens DC. 402,28 g/mL and Polygala paniculata L. 77,00 g/mL . Thin layer chromatography analysis shows that methanol extract of Polygala paniculata L. contains flavonoid.


Keywords


Cynara scolimus L., Artemisia china L., Borreria repens DC., Polygala paniculata L. DPPH, antioxidant



References

Amarowicz, R., Naczk, M., & Sahidi, F. 2000. Antioxidant activity of crude tannins of canola and rapeseed hulls, JAOCS, 77 : 957 – 961

Cervellati, R., Innocenti, G., Dall'Acqua, S., Costa, S., Sartini, E. 2004.Polyphenols from Polygala spp. and Their Antioxidant Activity, Chemistry & Biodiversity,1 (3) : 415-425

Harborne, J.B. 1987. Metode Fitokimia : Penentuan Cara Modern Menganalisis Tumbuhan Edisi 2, diterjemahkan oleh Padmawinata K. & Sudiro I., ITB, Bandung

Hart, H., Craine, L.E., Hart, D.J. 2003.Kimia Organik : Suatu Kuliah Singkat, diterjemahkan oleh Suminar Setiati Achmadi, Erlangga, Jakarta

Hegnauer, R. 1964.Chemotaxonomie der Pflanzen, Band 3, 447-545, Birkhauser Verlag, Stuttgart

Kikuzaki, H., Hisamoto, M., Hirose, K., Akiyama, K., and Taniguchi, H. 2002. Antioxidant properties of ferulic acid and its related compounds, J. Agric. Food Chem 50: 2161-2168

Markham, K. R. 1988.Cara Mengidentifikasi Flavonoid, diterjemahkan oleh Padmawinata. K., Penerbit ITB, Bandung

Molyneux, P. 2004. The use of stable free radical diphenylpicrylhidrazil (DPPH) for estimating antioxidant activity, Songklanakrin J.Sci.Technol.26 (2) : 211-219

Osawa, T., & Namiki, M. 1981. A Novel Type of Antioxidant Isolated from Leaf Wax of Eucalyptus leaf, Agric. Biol. Chem45 (3): 735-739

Rahayu, S. 2009. Penentuan Aktivitas Antioksidan dari Ekstrak Etanol Daun Ketapang (Terminalia catapa L.) dengan Metode 1,1-difenil-2-pikrilhidarzil, Tugas Akhir, Jurusan Kimia FMIPA UNDIP, Semarang

Rohman, A. & Riyanto, S. 2005. Antioxidant activity of mengkudu (Morinda citrifolia L.) fruit extract, Agritech 25 (3): 131-136

Sunarni, T., Pramono, S., Asmah, R. 2007. Flavonoid antioksidan penangkap radikal dari daun kepel (Stelechocarpus burahol (Bl.) Hook f. & Th.), M.F.I., 18 (3) : 111-116



DOI: https://doi.org/10.22146/tradmedj.8011

Article Metrics

Abstract views : 1920 | views : 18290

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2015 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada