.png)
.png)
* Corresponding Author
The Effect of Ethanolic Extract of Pandanus tectorius Leaves on Spatial Memory Ability and GCMS Analysis of Potentially Therapeutic Compounds
https://doi.org/10.22146/mot.79828 Yohana Krisostoma Anduk Mbulang(1*), Magi Melia Tanggu Rame(2), Cahyani Purnasari(3), Aloisius Masan Kopong(4)
(1) Undergraduate Program of Pharmacy, Universitas Citra Bangsa, Kupang, Nusa Tenggara Timur
(2) Undergraduate Program of Pharmacy, Universitas Citra Bangsa, Kupang, Nusa Tenggara Timur
(3) Undergraduate Program of Pharmacy, Universitas Citra Bangsa, Kupang, Nusa Tenggara Timur
(4) Study Program of Chemistry, Universitas Katolik Widya Mandira, Kupang, Nusa Tenggara Timur
(*) Corresponding Author
Abstract
Free radicals are known to damage the hippocampus and will decrease spatial memory. This condition will decrease cognitive function. This study focused on discovering the ability of the ethanolic extract of Pandanus tectorius leaves (EEPTL) to increase spatial memory and its active compounds using GC-MS. This study used twenty-five male mice (Mus musculus) divided into five groups. Three groups were given EEPTL orally using 100, 200, and 300mg/kg BW doses; one negative control (ethanol 10%) and one positive control (donepezil 0.65mg/kg BW). Each group was induced using ethanol 10% for three weeks (0.5mL/day) and tested for spatial memory using the Y-maze method. The result showed that EEPTL significantly increases mice's spatial memory ability (P<0.005). The most effective EEPTL dose is 300 mg/kg BW. GC-MS results showed that some active compounds in EEPTL are palmitic acid, stearic acid, coumarin, and 3-benzoyl-4-phenyl-ethyl linoleic. These results indicate that EEPTL increases spatial memory and contain several fatty acids, volatile oil, and phenols.
Keywords
Full Text:
PDFReferences
Ahmed, M.M., Wang, A.C.J., Elos, M., Chial, H.J., Sillau, S., Solano, D.A., Coughlan, C., Aghili, L., Anton, P., Markham, N. and Adame, V., (2021). Pro-inflammatory Cytokine GM-CSF Improves Learning/Memory and Brain Pathology in Dp16 Down Syndrome Mice and Improves Learning/Memory in Wild-Type Mice. BioRxiv.
Ayaz, M., Sadiq, A., Junaid, M., Ullah, F., Ovais, M., Ullah, I., Ahmed, J. and Shahid, M., (2019). Flavonoids as prospective neuroprotectants and their therapeutic propensity in aging-associated neurological disorders. Frontiers in aging neuroscience, 11, p.155.
Bhatt, P.R. and Bhatt, K.P., 2015. Neurobehavioral activity of Pandanus tectorius Parkinson (Pandanaceae) leaf extract in various experimental models. Journal of Pharma sci. Tech, 5(1), pp.16-18.
Du, A., Dai, X., Dong, J., Liu, J., Zhang, Y., Fu, P., Qin, H., Li, R. & Zhang, R. (2019). Effects of aminooxy acetic acid on hippocampal mitochondria in rats with chronic alcoholism the analysis of learning and memory-related genes. Journal of Integrative Neuroscience, 18(4), 451–462.
Crews, F.T. and Boettiger, C.A., 2009. Impulsivity, frontal lobes, and risk for addiction. Pharmacology Biochemistry and Behavior, 93(3), pp.237-247.
Hosseinzadeh, Z., Moazedi, A.A. and Chinipardaz, R., 2007. The effect of palmitic acid on spatial learning and extinction in the adult male rat. Pakistan Journal of Biological Sciences, 10(16), pp.2653-2658.
Kissling, J., Ioset, J.R., Marston, A. and Hostettmann, K., 2005. Bio‐guided isolation of cholinesterase inhibitors from the bulbs of Crinum × powellii. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 19(11), pp.984-987.
Kumar, N.R., Padmalaxmi, D., Udupa, A.L., Ojeh, N., Gupta, S., Rathnakar, U.P., Patil, V., Prabhath, G.K., Benegal, D., Benegal, A. and Rao, S.R., (2011). Antioxidant activity of methanol extract of Pandanus fascicularis Lam. Pharmacologyonline, 1, pp.833–841.
Omodamiro, O. and Ikekamma, C., 2016. In vitro study of antioxidant and anticoagulant activities of ethanol extract of Pandanus tectorius leaves. International Blood Research Reviews, 5(1), pp.1-11.
Suswanti, I., Budiharsana, M.P., Turana, Y. and Handajani, Y.S., 2020. Faktor Risiko Gangguan Kognitif Pada Lansia: Studi Prospektif Atma Jaya Active Aging Research. Jurnal Publikasi Kesehatan Masyarakat Indonesia, 7(1).
Tan, M.A., Ishikawa, H. and An, S.S.A., 2022. Pandanus amaryllifolius Exhibits In Vitro Anti-Amyloidogenic Activity and Promotes Neuroprotective Effects in Amyloid-β-Induced SH-SY5Y Cells. Nutrients, 14(19), 3962.
Toyokuni, S., (1999). Reactive oxygen species‐induced molecular damage and its application in pathology. Pathology international, 49(2), pp.91–102.
Vauzour, D., Vafeiadou, K., Rodriguez-Mateos, A., Rendeiro, C. and Spencer, J.P., 2008. The neuroprotective potential of flavonoids: a multiplicity of effects. Genes & nutrition, 3(3), pp.115-126.
Wang, Z.J., Liang, C.L., Li, G.M., Yu, C.Y. and Yin, M., 2007. Stearic acid protects primary cultured cortical neurons against oxidative stress. Acta Pharmacologica Sinica, 28(3), pp.315-326.
Y Yunus, J. and Sari, D.C.R., 2012. Efek Neuroprotektif Vitamin D3 terhadap Jumlah Sel Purkinje Cerebellum yang Diinduksi Etanol. Mutiara Medika: Jurnal Kedokteran dan Kesehatan, 12(2), pp.63-71.
Yusufzai, S.K., Khan, M.S., Sulaiman, O., Osman, H. and Lamjin, D.N., 2018. Molecular docking studies of coumarin hybrids as potential acetylcholinesterase, butyrylcholinesterase, monoamine oxidase A/B, and β-amyloid inhibitors for Alzheimer's disease. Chemistry Central Journal, 12(1), pp.1-57.
DOI: https://doi.org/10.22146/mot.79828
Article Metrics
Abstract views : 1315
| 
views : 1457
Refbacks
- There are currently no refbacks.
Copyright (c) 2023 Majalah Obat Tradisional
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Faculty of Pharmacy
Universitas Gadjah Mada