Phytochemical Screening and Antidengue Activity of Jatropha Multifida Extract against DENV-2

https://doi.org/10.22146/mot.73368

Mia Munawaroh Yuniyanti(1*), Dwi Aris Agung Nugrahaningsih(2), Mae Sri Hartati Wahyuningsih(3)

(1) - Magister of Biomedical Sciences, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta - Department of Pharmacology and Therapy, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta
(2) - Department of Pharmacology and Therapy, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta - Herbal Medical Center, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta
(3) - Department of Pharmacology and Therapy, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada - Herbal Medical Center, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Cases of dengue infection are still high in various parts of the world and no antiviral has been found to treat dengue infection. Jatropha multifida is one of the herbs used by the community to treat dengue infection, but pre-clinical and clinical scientific evidence has not been carried out. Phytochemical screening of ethanol extract of Jatropha multifida leaves was carried out using thin layer chromatography. Cytotoxic assay was performed on Vero cells using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide). The cytotoxic concentration 50 (CC50) was determined using probit analysis. Anti-dengue test on dengue  virus serotype 2 (DENV-2) was performed on Vero cells and the RNA (ribonucleic acid) copy number was quantified using quantitative polymerase chain reactions ((qPCR). Phytochemical screening results showed that the ethanol extract of Jatropha multifida leaves contains flavonoid and terpenoid compounds. CC50 of the extract was found to be 651.8 µg/mL. The RNA copy number of the treated group was lower than the control group and the difference was significant. The ethanol extract of Jatropha multifida leaves has anti-dengue activity against DENV-2.


Keywords


Jatropha multifida; dengue; phytochemical screening; DENV-2

Full Text:

PDF


References

Anani K, Adjrah Y, Ameyapoh Y, Karou S, Agbonon A, De Souza C, et al. Antimicrobial, Anti-inflammatory and antioxidant activities of Jatropha multifida L. (Euphorbiaceae). Pharmacognosy Res. 2016;8(2):142–6.

WHO. Dengue and Severe Dengue [Internet]. 2020 [cited 2021 Oct 10]. Available from: https://www.who.int/news-room/fact-sheets/detail/dengue-and-severe-dengue

Vaughn DW, Green S, Kalayanarooj S, Innis BL, Nimmannitya S, Suntayakorn S, et al. Dengue viremia titer, antibody response pattern, and virus serotype correlate with disease severity. J Infect Dis. 2000;181(1):2–9.

Low JGH, Ooi EE, Vasudevan SG. Current status of dengue therapeutics research and development. J Infect Dis. 2017;215(Suppl 2):S96–102.

Hamza OJM, van den Bout-van den Beukel CJP, Matee MIN, Moshi MJ, Mikx FHM, Selemani HO, et al. Antifungal activity of some Tanzanian plants used traditionally for the treatment of fungal infections. J Ethnopharmacol. 2006;108(1):124–32.

Juniarti, Aryeni, Poerwaningsih Y, Hernawati E, Jusuf, Aulia A, et al. Effects of Methanolic Jatropha multifida L. Extract in Wound Healing Assessed by the Total Number of PMN Leukocytes and Fibroblasts. MAKARA Sci Ser. 2013;16(3):178–82.

Sundaryono A, Listiono AE, Jumika R, Yahya R. Potential Test Development of Dengue Hemorrhagic Fever Medicine from Jatropha Multifida Stem Bark as Organic Chemistry Teaching Material. J Phys Conf Ser. 2019;1233(1).

Rampadarath S, Puchooa D, Ranghoo-Sanmukhiya VM. A comparison of polyphenolic content, antioxidant activity and insecticidal properties of Jatropha species and wild Ricinus communis L. found in Mauritius. Asian Pac J Trop Med. 2014;7(S1):S384–90.

Sabandar CW, Ahmat N, Jaafar FM, Sahidin I. Medicinal property, phytochemistry and pharmacology of several Jatropha species (Euphorbiaceae): A review. Phytochemistry [Internet]. 2013;85:7–29. Available from: http://dx.doi.org/10.1016/j.phytochem.2012.10.009

Boniface PK, Ferreira EI. Flavonoids as efficient scaffolds: Recent trends for malaria, leishmaniasis, Chagas disease, and dengue. Phyther Res. 2019;33(10):2473–517.

Lim SYM, Chieng JY, Pan Y. Recent insights on anti-dengue virus (DENV) medicinal plants: review on in vitro, in vivo and in silico discoveries. All Life [Internet]. 2021;14(1):1–33. Available from: https://doi.org/10.1080/26895293.2020.1856192

Tohma D, Tajima S, Kato F, Sato H, Kakisaka M, Hishiki T, et al. An estrogen antagonist, cyclofenil, has anti-dengue-virus activity. Arch Virol [Internet]. 2019;164(1):225–34. Available from: https://doi.org/10.1007/s00705-018-4079-0

Ellan K, Thayan R, Raman J, Hidari KIPJ, Ismail N, Sabaratnam V. Anti-viral activity of culinary and medicinal mushroom extracts against dengue virus serotype 2: An in-vitro study. BMC Complement Altern Med. 2019;19(1):1–12.

Gurukumar K, Priyadarshini D, Patil J, Bhagat A, Singh A, Shah P, et al. Development of real time PCR for detection and quantitation of Dengue Viruses. Virol J. 2009;6:1–8.

Thomas S, Sallykutty Thomas C. Pharmacognostic and phytochemical constituents of leaves of Jatropha multifida Linn. and Jatropha podagrica Hook. J Pharmacogn Phytochem. 2016;5(2):243–6.

Zhu JY, Zhang CY, Dai JJ, Rahman K, Zhang H. Diterpenoids with thioredoxin reductase inhibitory activities from Jatropha multifida. Nat Prod Res [Internet]. 2017;31(23):2753–8. Available from: http://dx.doi.org/10.1080/14786419.2017.1297441

Phuwajaroanpong A, Chaniad P, Horata N, Muangchanburee S, Kaewdana K, Punsawad C. In Vitro and In Vivo Antimalarial Activities and Toxicological Assessment of Pogostemon Cablin (Blanco) Benth. J Evidence-Based Integr Med. 2020;25:1–8.

Werdyani S, Fitria A, Rakhmawati S. In Vitro Tracing of Cytotoxic Compounds in Jarak Cina Stem Bark (Jatropha Multifida Linn.). EKSAKTA J Sci Data Anal. 2020;1(1):7–13.

Rivera J, Rengifo AC, Sarmiento L, Díaz T, Laiton-Donato K, Gracia M, et al. Nuclei ultrastructural changes of C6/36 cells infected with virus dengue type 2. Biomedica. 2018;38:135–43.

Zargar S, Wani TA, Jain SK. Morphological changes in vero cells postinfection with dengue virus type-2. Microsc Res Tech. 2011;74(4):314–9.

Peng M, Watanabe S, Chan KWK, He Q, Zhao Y, Zhang Z, et al. Luteolin restricts dengue virus replication through inhibition of the proprotein convertase furin. Antiviral Res. 2017;143:176–85.

Kaushik S, Dar L, Kaushik S, Yadav JP. Identification and characterization of new potent inhibitors of dengue virus NS5 proteinase from Andrographis paniculata supercritical extracts on in animal cell culture and in silico approaches. J Ethnopharmacol [Internet]. 2021;267(August 2020):113541. Available from: https://doi.org/10.1016/j.jep.2020.113541



DOI: https://doi.org/10.22146/mot.73368

Article Metrics

Abstract views : 1519 | views : 1827

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2022 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada