Metabolomic Study of Three Species in Zingiberaceae Family based on 1H-NMR

https://doi.org/10.22146/mot.41071

Dinar Sari Cahyaningrum Wahyuni(1*), Magdalena Kristanti(2), Soerya Dewi Marliyana(3), Yudi Rinanto(4)

(1) Program Studi Farmasi, Universitas Sebelas Maret
(2) Program Studi Farmasi, Universitas Sebelas Maret
(3) Program Studi Kimia, FMIPA, Universitas Sebelas Maret
(4) Pendidikan Biologi, FKIP, Universitas Sebelas Maret
(*) Corresponding Author

Abstract


Zingiberaceae is an economical plant and widely used as traditional medicines. Its rhizomes have been reported to have biological effects due to their metabolites content. However, metabolites profiling in Zingiberaceae has been little reported comprehensively. Therefore, this study aimed to profile primary and secondary metabolite of three species Zingiberaceae rhizomes (Zingiber amaricans BI, Zingiber officinale Roscoe, and Alpinia purpurata (Vieill.) K.Schum using 1H-NMR-based metabolomic approach. All samples were collected from local farmer located in Nguter, Sukoharjo, Central Java. Multivariate statistical analysis and ANOVA applied to measure the differences. It resulted that metabolite profiling discriminated between Zingiber and Alpinia samples. Fructose, α-glucose, β-glucose, sucrose, malic acid, alanine, valine, and shogaol contributed in discrimination between Z. amaricans BI, Z. officinale Roscoe, and A. purpurata (Vieill.) K.Schum. Sugar (α-glucose, β-glucose, fructose, and sucrose) and malic acid were significantly higher in Alpinia than in Zingiber samples. Relative concentration of amino acids (alanine and valine) and shogaol were significantly higher in Z. officinale. This result might be useful for databases and supplementary informations in Z. amaricans, Z. officinale, and A. purpurata taxonomy classifications.

Keywords


Zingiberaceae; Z. amaricans BI; Z. officinale Roscoe; A. purpurata (Vieill.) K.Schum; metabolomic; 1H-NMR

Full Text:

PDF


References

Ali, Kashif, Federica Maltese, Ana Margarida Fortes, et al. 2011. “Monitoring Biochemical Changes during Grape Berry Development in Portuguese Cultivars by NMR Spectroscopy.” Food Chemistry 124(4):1760–69.

Ali, Kashif, Federica Maltese, Reinhard Toepfer, Young Hae Choi, and Robert Verpoorte. 2011. “Metabolic Characterization of Palatinate German White Wines According to Sensory Attributes, Varieties, and Vintages Using NMR Spectroscopy and Multivariate Data Analyses.” Journal of Biomolecular NMR 49(3–4):255–66.

Chan, Eric Wei Chiang and Siu Kuin Wong. 2015. “Phytochemistry and Pharmacology of Ornamental Gingers, Hedychium Coronarium and Alpinia Purpurata: A Review.” Journal of Integrative Medicine 13(6):368–79.

Fernie, Alisdair R., Richard N. Trethewey, Arno J. Krotzky, and Lothar Willmitzer. 2004. “Metabolite Profiling: From Diagnostics to Systems Biology.” Nature Reviews Molecular Cell Biology 5:763.

Gomez-Casati, Diego F., Maria I. Zanor, and María V Busi. 2013. “Metabolomics in Plants and Humans: Applications in the Prevention and Diagnosis of Diseases.” BioMed Research International 2013.

Jiang, Hongliang et al. 2006. “Metabolic Profiling and Phylogenetic Analysis of Medicinal Zingiber Species: Tools for Authentication of Ginger (Zingiber Officinale Rosc.).” Phytochemistry 67(15):1673–85.

Karuppiah, Ponmurugan and Shyamkumar Rajaram. 2012. “Antibacterial Effect of Allium Sativum Cloves and Zingiber Officinale Rhizomes against Multiple-Drug Resistant Clinical Pathogens.” Asian Pacific Journal of Tropical Biomedicine 2(8):597–601.

Keong, YEAP SWEE et al. 2010. “Immunomodulatory Effects of Zerumbone Isolated from Roots of Zingiber Zerumbet.” Pak J Pharm Sci 23(1):75–82.

Kim, Hye Kyong, Young Hae Choi, and Robert Verpoorte. 2010a. “NMR-Based Metabolomic Analysis of Plants.” Nature Protocols 5(3):536–49.

Kim, Hye Kyong, Young Hae Choi, and Robert Verpoorte. 2010b. “NMR-Based Metabolomic Analysis of Plants.” Nature Protocols 5(3):536.

Kochuthressia, K. P., S. John Britto, M. O. Jaseentha, L. Joelri Michael Raj, and S. R. Senthilkumar. 2010. “Antimicrobial Efficacy of Extracts from Alpinia Purpurata (Vieill.) K. Schum. against Human Pathogenic Bacteria and Fungi.” Agriculture and Biology Journal of North America 1(6):1249–52.

Krishnan, P., N. J. Kruger, and R. G. Ratcliffe. 2004. “Metabolite Fingerprinting and Profiling in Plants Using NMR.” Journal of Experimental Botany 56(410):255–65.

Kumar, Gaurav, L. Karthik, and K. V. Bhaskara Rao. 2011. “A Review on Pharmacological and Phytochemical Properties of Zingiber Officinale Roscoe (Zingiberaceae).” Journal of Pharmacy Research 4(9):2963–66.

Lee, Jang-Eun et al. 2015. “Metabolomic Unveiling of a Diverse Range of Green Tea (Camellia Sinensis) Metabolites Dependent on Geography.” Food Chemistry 174:452–59.

Lee, Min Young et al. 2016. “Discrimination of Polygonatum Species and Identification of Novel Markers Using 1H NMR‐and UPLC/Q‐TOF MS‐based Metabolite Profiling.” Journal of the Science of Food and Agriculture 96(11):3846–52.

Raj, Chinthamony Arul, Paramasivam Ragavendran, Dominic Sophia, Muthaiyan Ahalliya Rathi, and Velliyur Kanniappan Gopalakrishnan. 2012. “Evaluation of in Vitro Antioxidant and Anticancer Activity of Alpinia Purpurata.” Chinese Journal of Natural Medicines 10(4):263–68.

Sang, Shengmin et al. 2009. “Increased Growth Inhibitory Effects on Human Cancer Cells and Anti-Inflammatory Potency of Shogaols from Zingiber Officinale Relative to Gingerols.” Journal of Agricultural and Food Chemistry 57(22):10645–50.

Santos, Geanne K. N. et al. 2012. “Essential Oils from Alpinia Purpurata (Zingiberaceae): Chemical Composition, Oviposition Deterrence, Larvicidal and Antibacterial Activity.” Industrial Crops and Products 40:254–60.

Thomson, M. et al. 2002. “The Use of Ginger (Zingiber Officinale Rosc.) as a Potential Anti-Inflammatory and Antithrombotic Agent.” Prostaglandins, Leukotrienes and Essential Fatty Acids 67(6):475–78.

Villaflores, Oliver B. et al. 2010. “Phytoconstituents from Alpinia Purpurata and Their in Vitro Inhibitory Activity against Mycobacterium Tuberculosis.” Pharmacognosy Magazine 6(24):339.

Watkins, Steven M. and J. Bruce German. 2002. “Metabolomics and Biochemical Profiling in Drug Discovery and Development.” Current Opinion in Molecular Therapeutics 4(3):224–28.



DOI: https://doi.org/10.22146/mot.41071

Article Metrics

Abstract views : 5652 | views : 2942

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2019 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada