Antinociceptive Activity of Aqueous Fraction of Kratom Leaves Mitragyna speciosa Korth.) on Male Swiss Albino Mice

https://doi.org/10.22146/mot.32085

Widia Indri Nugraha(1), Robiyanto Robiyanto(2), Sri Luliana(3*)

(1) Faculty of medicine Tanjungpura university
(2) Faculty of medicine Tanjungpura university
(3) Faculty of medicine Tanjungpura university
(*) Corresponding Author

Abstract


Kratom (Mitragyna speciosa Korth.) has been known to have an analgesic opioid effect (antinociceptive). The major compound of kratom leaf is mitraginin, which has strong affinity on opioid receptor. The aim of this research is to prove antinociceptive effect of aqueous fraction of kratom leaf and its effective dose. The simplicia of kratom leaf was extracted with methanol 96%. Methanol extract fractioned with n-hexane, dichloromethane, ethyl acetate, and aquadest. This research used hot plate method on male Swiss Albino mice. The subject divided to 5 groups, negative control group, morphine 5,46 mg/kgBW, aqueous fraction 140, 240 and 560 mg/kgBW. Latency time was measured every 15 minutes over 2 hours period. Analytic statistical of latency time using One Way ANOVA shows that the aqueous fraction at the dose of 140, 280 and 560 mg/kgBW significantly differentiate with negative control group and positive control group. The antinociceptive effect increases with increasing doses. The three doses showed that the antinociceptive effect was no better than the positive control (Morphine)

Keywords


antinociceptive; aqueous fraction; Mitragyna speciosa; morphine

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References

Ahmad, K. & Aziz, Z., 2012. Mitragyna speciosa use in the northern states of Malaysia : A cross-sectional study. Journal of Ethnopharmacology, 141(1), pp.446–450.

Gulnur Toker, Esra Kupeli, Merve Memisoglu, E.Y., 2004. Flavonoids with antinociceptive and anti-inflammatory activities from the leaves of Tilia argentea ( silver linden ). Journal of Ethnopharmacology, 95, pp.393–397.

Gunawan SG, Setiabudy R, Nafrialdi, Elysabeth. Farmakologi dan terapi. Edisi V. Jakarta: Badan Penerbit FKUI; 2012.

Jace A. Daniels, 2015. MITRAGYNA SPECIOSA : AN ANALYTICAL STUDY. [Thesis]. The University of Alabama at Birmingham.

Jayanthi MK, Jyoti MB., 2012. Experimental animal studies on analgesic and anti-nociceptive activity of Allium sativum ( Garlic ) powder. The Indian Journal of Research and Reports in Medical Sciences, 2(1), pp.1–6.

Khan, H., Muhammad Saeed, Anwal-Ul-Hassan Gilani, M.A.K. & Ahsana Dar, I.K., 2010. The antinociceptive activity of Polygonatum verticillatum rhizomes in pain models. Journal of Ethnopharmacology, 127, pp.521–527.

Lesiak AD, Cody RB, Dane AJ, Musah R., 2014. Rapid detection by direct analysis in real time-mass spectrometry ( DART-MS ) of psychoactive plant drugs of abuse : The case of Mitragyna speciosa aka “‘ Kratom .’” Forensic Science International, 242, pp.210–218.

Matsumoto, K., Syunji Horie, Hayato Ishikawab, H.T., Norio Aimi, Dhavadee Ponglux, Kazuo W., 2004. Antinociceptive effect of 7-hydroxymitragynine in mice : Discovery of an orally active opioid analgesic from the Thai medicinal herb Mitragyna speciosa. Life Sciences, 74, pp.2143–2155.

Mazumder, U.K., Gupta, M. & Rath, N., 1998. CNS Activities of Cassia fistula in Mice. Phytotherapy Research, 12(April), pp.520–522.

Muhammad, N., 2014. In-Vivo Models for Management of Pain. Pharmacology & Pharmacy, 5, pp.92–96.

N.F Idayu, M. Taufik Hidayat, M.A.M.Moklas, F.Sharida, A.R.Nurul Raudzah, A.R.Shamma, and Evhy.A., 2011. Antidepressant-like effect of mitragynine isolated from Mitragyna speciosa Korth in mice model of depression. Phytomedicine, 18, pp.402–407.

Pal, D., Sannigrahi, S. & Mazumder, U.K., 2009. Analgesic and anticonvulsant effects of saponin isolated from the leaves of Clerodendrum infortunatum Linn . in mice. Indian Journal of Experimental Biology, 47, pp.743–747.

Parthasarathy, S., Juzaili Bin Azizi, Surash Ramanathan, S.I. & Sreenivasan Sasidharan, M.I.M.S. and S.M.M., 2009. Evaluation of Antioxidant and Antibacterial Activities of Aqueous, Methanolic and Alkaloid Extracts from Mitragyna Speciosa (Rubiaceae Family) Leaves. Molecules, 14, pp.3964–3974.

R. N. Suresha, S. et al., 2014. Evaluation of analgesic activity of perindopril in albino mice. Journal Advanced Pharmaceuutical Technology & Research, 5(3), pp.129–134.

Reanmongkol, W., Keawpradub, N. and Sawangjaroen, K., 2007. Effects of the extracts from Mitragyna speciosa Korth . leaves on analgesic and behavioral activities in experimental animals. Journal Science Technology, 29, pp.39–48.

Sabetghadam, A., Ramanathan, S. and Mansor, S.M., 2010. The evaluation of antinociceptive activity of alkaloid , methanolic , and aqueous extracts of Malaysian Mitragyna speciosa Korth leaves in rats. Pharmacognosy Research, 2(3), pp.181–185.

Sanagi M, Fauze MM, Norashidah O, Wan Aini Wan Ibrahim and Iqbal Hussaina, 2013. Determination of mitragynine for the identification of mitragyna species in Kedah ( Malaysia ) by gas chromatography-mass spectrometry. Der Pharma Chemica, 5(5), pp.131–138.

Shamima, A.R., Fakurazi, S. and Hidayat, M.T., 2012. Antinociceptive Action of Isolated Mitragynine from Mitragyna Speciosa through Activation of Opioid Receptor System. International Journal of Molecular Sciences, 251, pp.11427–11442.

W.M. Shaik Mossadeq, M.R. Sulaiman, T.A. Tengku Mohamad, H.S. Chiong, Z.A. Zakaria, M.L. Jabit, M.T.H. Baharuldin, D.A.I., 2009. Anti-Inflammatory and Antinociceptive Effects of Mitragyna speciosa Korth. Medical Principles and Practice, 18, pp.378–384.

Yeni Ridayani, 2013. uji efek sedatif fraksi etanol daun kratom (Mitragyna speciosa Korth.) pada mencit Jantan Galur BALB/c. IPI jurnal Mahasiswa Farmasi Fakultas Kedokteran dan Ilmu kesehatan UNTAN, 3, pp.1–9.




DOI: https://doi.org/10.22146/mot.32085

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