The Effectiveness Test of Oil Phase Ointment Containing Snakehead Fish (Channa striata) Extract on Open Stage II Acute Wounded Wistar Strain Male Rats

https://doi.org/10.22146/tradmedj.27920

Fransisca Daisa(1*), Mohamad Andrie(2), Wintari Taurina(3)

(1) Program Study of Pharmacy, Faculty of Medicine, Tanjungpura University, Pontianak
(2) Program Study of Pharmacy, Faculty of Medicine, Tanjungpura University, Pontianak
(3) Program Study of Pharmacy, Faculty of Medicine, Tanjungpura University, Pontianak
(*) Corresponding Author

Abstract


The snakehead (Channa striata) contained fatty acids omega-3 and omega-6 are useful as nutrients in accelerating the wound healing process. This study aims to determine the effectiveness of the oil phase extract ointment snakehead in the healing of acute wounds open stage II. Testing the effectiveness of wound healing using 6 groups: normal, gel bioplacenton (positive control), ointment base (negative control), ointments snakehead extract oil phase concentration of 10; 20 and 40% of the 4 rats with acute wounds open stage II and observation until day 16 injury. The area of the wound was measured with the image J program Macbiophotonic, calculated% healing power, and AUC values. A statistical test to the total AUC values per rat with SPSS for Windows 22.0 program using One Way ANOVA and Post-hoc LSD test. The results showed a significant difference in the negative group with ointment concentration of 20% (p <0.05). The treatment group phase ointment fish oil extract is effective for wound healing is best to have a concentration of 20% for percentage of wound healing power of 97.157% and the average AUC value of 865.683% of the day.

Keywords


Salep; fase minyak ekstrak; ikan gabus; penyembuhan luka; Macbiophotonic Image J; Ointment; fatty acid; snakehead oil; wound healing; Macbiophotonic image J

Full Text:

PDF


References

Andrie, M. 2015. Formulasi Salep Ekstrak Ikan Gabus (Channa striata) dengan Variasi Konsentrasi Basis. Penelitian Dosen Dana DIPA, Pontianak, Universitas Tanjungpura.

Ansel, H.C. 1989. Pengantar Bentuk Sediaan Farmasi. 4th ed (Penterjemah: Farida Ibrahim), UI Press, Jakarta, pp. 490-494.

Anton, T.J. 1989. Occupational Safety and Health Management, McGraw-Hill Book.Co, Singapore, pp. 118-121.

Astawan, M. 1998. Teknik ekstraksi dan pemanfaatan minyak ikan untuk kesehatan. Buletin Teknologi dan Industri Pangan, pp. 9:44-51.

Carville, K. 2012. Wound Care Manual. 6th ed, Silver Chain Foundation, Western Australia.

Collins, N., and Sulewski, C. 2011. Ostomy Wound Management, pp. 10-13.

Gadekar, R., Saurabh, M.K., Thakur, G.S., dan Saurabh, A. 2012. Studi of Formulation, Characterisation and Wound Healing Potential of Transdermal Patches of Curcumin. Asian J Pharm Clin Res, 5:225-230.

Gitarja, W.S. 2008. Perawatan Luka Diabetes, Wocare, Bogor, Indonesia.

Gusdi, O. 2012. Formulasi sediaan gel ekstrak ikan gabus (Channa Striata) sebagai obat luka sayat, Skripsi, Pontianak, Universitas Tanjungpura;.

Hankenson, K.D., Watkins, B.A., Schoenlein, I.A., Allen, K.G., Turek, J.J. 2000. Omega-3 fatty acids enhance ligament fibroblast collagen formation in association with changes in interleukin-6 production, Proc. Soc. Exp. Biol. Med, 223:88-95.

Indonesia Enterostomal Therapy Nurse Association (InETNA). 2004. Tim Perawatan Luka dan Stoma Rumah Sakit Dharmais. Perawatan luka, Makalah Mandiri, Jakarta, pp. 1-16.

Mansjoer, A. 2000. Kapita selekta kedokteran Edisi III, Media Aesculapius FKUI, Jakarta.

Monteiro, Ana, P.T et al. 2011. Leukotriene B4 Mediates Neutrophil Migration Induced by Heme. J Immunol, 186:6562-6567.

Morton, J.J.P., and Malone, M.H. 1992. Evaluation of Vulnerary by An Open Wound Procedure in Rats. Archieve Int Pharmacodyn, 196:117-128.

Naibaho, Yamlean, O.H., Wiyono, W. 2013. Pengaruh Basis Salep Terhadap Formulasi Sediaan Salep Ekstrak Daun Kemangi (Ocimum sanctum L.) pada Kulit Punggung Kelinci yang Dibuat Infeksi Staphylococcus aureus, Manado, Pharmacon Jurnal Ilmiah Farmasi, 2.

Naveh, H.R., Jafari, Taghavi, M.M., Shariati, M., Vazeirnejad, R., dan Rezvani, M.E. 2011. Both omega-3 and omega-6 polyunsaturated fatty acids stimulate foot wound healing in chronic diabetic rat, Afr. J. Pharm. Pharmacol, 5:1713-1717.

Sabale, P., Bhimani, B., Prajapati, C., Sabale, V. 2012. An Overview of Medicinal Plants as Wound Healers, J App Pharm Sci, 2:143-150.

Saleh, M., Hari, E.I., Delima, H.D., dan Siswoputranto, P.S. 1985. Standar tepung ikan di dalam pengembangan industri tepung ikan, Tim Analisa Komoditi Sekretariat Jenderal Departemen Pertanian, pp. 1-7.

Serhan, C.N. 2007. Resolution Phase of Inflammation: Novel Endogenous Anti-Inflammatory and Proresolving Lipid Mediators and Pathways. Annu. Rev. Immunol, 25:101–137.

Shafri, M.A., dan Mat, J.A.M. 2012. Therapeutic Potential of Haruan (Channa striatus): from food to medicinal uses, Mal. J. Nutr, 18:125-136.

Sinambela, H.Y. 2012. Optimasi formulasi sediaan salep minyak ikan gabus (Channa Striata Bloch) sebagai obat luka sayat dengan metode simplex lattice design, Skripsi, Pontianak, Universitas Tanjungpura.

Tarigan, R., Pemila, U. 2007. Perawatan Luka: Moist Wound Healing. Makalah, Jakarta, Universitas Indonesia.



DOI: https://doi.org/10.22146/tradmedj.27920

Article Metrics

Abstract views : 7035 | views : 10169

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2017 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada