ISOLATION OF 8-HIDROXY-6,7-DIMETHOXY COUMARIN FROM JARAK TINTIR STEM (Jatropha multifida L.) AND ITS TOXICITY VALUE USING BRINE SHRIMP LETHALITY TEST (BSLT)

https://doi.org/10.22146/tradmedj.24303

Akhirul Kahfi Syam(1*), Muhamad Insanu(2), Komar Ruslan Wirasutisna(3)

(1) Jenderal Achmad Yani University, Cimahi School of Pharmacy, Institut Teknologi Bandung
(2) School of Pharmacy, Institut Teknologi Bandung
(3) School of Pharmacy, Institut Teknologi Bandung
(*) Corresponding Author

Abstract


Jatropha multifida L. (jarak tintir) was a shrub, annual, and had ± 2 m high. Empirically jarak tintir sap was used as traditional medicine by Indonesian people for a long time. Only limited studies were conducted regarding its chemical compound. It was reported that multifidone (diterpenoid compound from the stem) had an activity against cancer cells in vitro. This study aimed to test the toxicity of various extracts (n-hexane, ethyl acetate, and methanol) with Brine Shrimp Lethality Test (BSLT) of Jatropha multifida L. stem. A Fraxidin (8-hidroxy-6,7-dimethoxy coumarin) has been isolated from ethyl acetate fraction based on highest cytotoxic with LC50 value 3.69 µg/mL. The isolated compound was elucidated to gain chemical structure base on spectroscopic data (UV-Vis Spectrofotometric, IR Spectrofotometric, and NMR). Toxicity of fraxidin was tested on BSLT and showed no potential activity with LC50 value > 500 μg/mL.

Keywords


Fraxidin; BSLT; coumarin; Jatropha multifida L.

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References

Das, B., Laxminarayana, K., Krishnaiah, M., Srinivas, Y. dan Raju, T. V. (2009): Multidione, A Novel Diterpenoid From Jatropha multifida, Tetrahedron Letters, 50 (34), 4885-4887.

Das, B., Ravikanth, B., Reddy, K. R., Thirupathi, P., Raju, T. V. dan Sridhar, B. (2008a): Diterpenoids from Jatropha multifida, Phytochemistry, 69 (14), 2639-2641.

Das, B., Reddy, K. R., Ravikanth, B., Raju, T. V., Sridhar, B., Khan, P. U. dan Rao, J. V. (2008b): Multifidone: A Novel Cytotoxic Lathyrane-Type Diterpene Having An Unusual Six-Membered A Ring From Jatropha multifida, Bioorganic & medicinal chemistry letters, 19 (1), 77-79.

Depkes (2000): Parameter Standar Umum Ekstrak Tumbuhan Obat, Direktorat Jenderal Pengawasan Obat dan Makanan Direktorat Pengawasan Obat Tradisional, Jakarta.

Farnsworth, N. (1966): Biological and Phytochemical Screening of Plants, Journal of Pharmaceutical Sciences, 55 (3), 243-268.

Honasan, Y. (2012): Isolasi dan Uji Toksisitas Senyawa Toksik Golongan Terpenoid dan Fenol Daun Jarak Tintir (Jatropha multifida Linn.) dengan Metode Brine Shrimp Lethality Test (BSLT), Skripsi, Jurusan Farmasi Fakultas MIPA Universitas Jenderal Achmad Yani, Cimahi.

Hutapea, J. (2000): Inventaris Tanaman Obat Indonesia, Departemen Kesehatan RI dan Badan Penelitian dan Pengembangan Kesehatan, Jakarta.

Kanth, B. S., Kumar, A. S., Shinde, D. B., Babu, K. H., Raju, T. V., Kumar, C. G., Sujitha, P. dan Das, B. (2011): New Bioactive Macrocyclic Diterpenoids From Jatropha multifida, Bioorganic & medicinal chemistry letters, 21 (22), 6808-6810.

McLaughlin dan Jerry, L. (1991): Crown Gall Tumours on Potato Discs and Brine Shrimp Lethality: Two Simples Bioassays for Higher Plant Screening and Fractination, Methods in Plant Biochemistry, 6, 8-10.

Meyer, B. N., Ferrigni, N. R., Putnam, J. E., Jacobsen, L. B., Nichols, D. E. dan McLaughlin, J. L. (1982): Brine Shrimp: A Convenient General Bioassay for Active Plant Constituents, Planta Medica, 45 (5), 31-34.

Murray, R. D. H., Mendez, J. dan Brown, S. A. (1982): The Natural Coumarins: Occurrence, Chemistry, and Biochemistry, John Wright & Sons, Bristol.

Pretsch, E., Buhlmann, P. dan Badertscher, M. (2009): Structure Determination of Organic Compounds, Springer, Berlin.

Rumzhum, N. N., Sohrab, M. H., Al-Mansur, M. A., Rahman, M. S., Hasan, C. M. dan Rashid, M. A. (2012): Secondary Metabolites from Jatropha podagrica Hook Journal of Physical Science, 23 (1), 29-37.



DOI: https://doi.org/10.22146/tradmedj.24303

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