QUALITY STANDARDIZATION OF BROTOWALI (Tinospora crispa) STEM EXTRACT

https://doi.org/10.22146/tradmedj.10672

Harwoko Harwoko(1*), Nur Amalia Choironi(2)

(1) Laboratory of Pharmaceutical Biology, Faculty of Health Sciences, Universitas Jenderal Soedirman, Jl. dr. Soeparno Karangwangkal Purwokerto 53123
(2) Laboratory of Pharmaceutical Biology, Faculty of Health Sciences, Universitas Jenderal Soedirman, Jl. dr. Soeparno Karangwangkal Purwokerto 53123
(*) Corresponding Author

Abstract


Brotowali (Tinospora crispa) has been traditionally used for the treatment of gout and scientifically reported as analgesic, anti-inflammatory, and antihyperuricemic agents. Tinospora crispa stem is one of herbal medicine material that its quality should be standardized. This study aims to determine the quality parameters of the T. crispa ethanolic extract included specific and non-specific parameters. Brotowali stem were macerated using ethanol 70%, then the non-specific parameters such as the water content, total ash, total contaminant number of bacteria and fungus were determined. The specific parameters including organoleptic properties, water soluble extract, ethanol soluble extract, and the thin layer chromatography (TLC) profile have also been determined. The parameter values were compared to the qualification of traditional medicine from Department of Health (Depkes R.I.). The result showed that T.crispa stem ethanolic extract has the water content was 8.12±0.06% and the total ash was 5.20 ± 0.12%. The microbiology results showed that the total contaminant of bacteria as much as 5 x 102 CFU/g and fungus as much as 5 x 103 CFU/g. This extract was brown viscous extract, bitter taste and characteristic odor with water soluble fraction was 45.09 ± 0.67% and ethanol soluble fraction was 14.19 ± 0.14%. The TLC profile of ethanolic extract indicates the existence of flavonoids and alkaloids. Total flavonoids of brotowali extract (32.65 ± 0.20%) rutin equivalent.


Keywords


Tinospora crispa, brotowali, quality standardization, standardized extract

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References

Amom, Z., Nautical, H., Ismail, S., Ismail, NA, Shah, ZM, and Arsyad, M.S. 2009. Nutritional Composition, Antioxidant Ability and Flavonoid Content of Tinospora crispa stem. Advances in Natural and Applied Scienhipoces . 3 (1): 88-94.

Chang, C., Yang, M., Wen, H. and Chem, J. 2002. Estimation of flavonoid total content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis. 10 (3): 178-182.

Chen, L., Yin, H., Lan, Z., Ma, S., Zhang, C., Yang, Z., Li, P., and Lin, B. 2011. Anti-hyperuricemic and nephroprotective effects of Smilax china L. Journal of Ethnopharmacology. 135: 399 -405.

Coss, P., Ying, L., Calomme, JP, Cimanga, K., Van Poel, B., Pieters, L., Vlietinck., AJ, and Vanden BD. 1998. Structure-Activity Relationship and Classification of Flavonoids as Inhibitors of Xanthine oxidase and superoxide Scavengers. Journal of Natural Product. 61 (1): 71-76.

Cotelle, N. 2001. Role of Flavonoids in Oxidative Stress. Current Topics in Medicinal Chemistry. 1 (6): 569-590.

Dalimartha, S. 2008. Resep Tumbuhan Obat untuk Asam Urat. Penebar Swadaya, Jakarta. pp 41- 42.

Department of Health. 1978. Materia Medika Indonesia. 2nd Edition. Departemen Kesehatan Republik Indonesia. Jakarta. pp 91- 95.

Department of Health. 2000. Parameter Standar Umum Ekstrak Tumbuhan Obat, Edisi I. Departemen Kesehatan Republik Indonesia, Jakarta. pp 9-12.

De Souza, M.R., de Paula, C.A., de Resende, M.L.P.,

Grabe-Guimaraes, A., Filho, J.D.S., and Saude-Guimaraes, D.A. 2012. Pharmacological basis for use of Lychnophora trichocarpha in gouty arthri tis: Anti-hyperuricemic and anti- inflammatory effects of its extract, fraction and constituents. Journal of Ethnopharmacology. 145: 845-850.

Dewoto, H.R. 2007. Pengembangan Obat Tradisional Indonesia Menjadi Fitofarmaka. Majalah Kedokteran Indonesia. 57 (7): 205- 211.

Handayani. 2010. Efek Antiangiogenik Ekstrak Kloroform Batang Tinospora crispa pada Membran Korio Alantoin Embrio Ayam Terinduksi bFGF. Indonesian Journal of Pharmacy. 2 (1): 124-128.

Harborne, J.B. 1996. Metode Fitokimia, Penuntun Cara Modern

Menganalisis Tumbuhan, translated by K. Padmawirata. dan I. Soediro. ITB Press, Bandung. p 69.

Hariyati, S. 2005. Standardization Extracts of Indonesian Medicinal Plant, One of The Important Stages in the Dev elopment of Indonesia Traditional Medicine. Info POM. 6 (4): 1-5.

Harwoko, Warsinah, and Utami, E.D. 2015. Antihyperuricemic activity of Tinospora crispa purified extrac t on potassium oxonate induced mice. Proceeding of

Hipol, R.L., Nenette, M.F., and Hipols, R.M. 2012. Antiinflamatory Activities of the Aqueous Extract of The Stem of Tinospora crispa (Family Menispermaceae). Journal of Nature Studies. 11 (1&2): 88-95.

Irianti, T., Puspitasari, A., and Suryani, E. 2011. Aktivitas Penangkapan Radikal 2,2-Difenil- 1-Pikrilhidrazil oleh Ekstrak Etanolik Batang Brotowali (Tinospora crispa (L.) Miers) dan Fraksi-Fraksinya. Majalah Obat Tradisional. 16 (3): 138-144.

Kneifel, W., Czech, E., and Kopp, B. 2002. Microbial Contamination of Medicinal Plants - A Review. Planta Medica. 68 (1): 5-15.

Mansour, E.H and Khalil, A H. 2000. Evaluation of Antioxidant

Activity of Some Plant Extracts and Their Application to Ground Beef Patties. Food Chemistry. 69: 135-41.

Ministry of Health. 2012. Indonesian Herbal Pharmacopeia. Kementerian Kesehatan Republik Indonesia. Jakarta, pp 368-370.

Mutiatikum, D., Raini, M., and Lastari, P. 2004. Uji Mutagenesis dan Karakterisasi Batang Brotowali (Tinospora tuberculata), Media Litbang Kesehatan, XIV (1): 22-27.

Sudarsono, P.A., Gunawan, D., Wahyuono, S., Donatus, I.A., Dradjad, M., Wibowo, S., and Ngatidjan. 2006. Tumbuhan Obat. Pusat Penelitian Obat Tradisional (PPOT-UGM), Yogyakarta. pp 144-149.

Sulaiman M. R., Zakaria Z. A. and Lihan R. 2008. Antinociceptive and Anti -inflammatory Activities of Tinospora crispa in Various Animal Models. International Journal of Tropical Medicine. 3 (3): 66-69.

Wagner, H. and Bladt, S. 1996. Plant Drug Analysis, A Thin Layer Chromatography Atlas, 2nd Edt.. Springer, Berlin Heidelberg. pp 324-325.

Wahyuningsih, M.S.H. 2006. Deskriptif Penelitian Dasar Herbal Medicine. Majalah Obat Tradisional. 11 (38): 7-12



DOI: https://doi.org/10.22146/tradmedj.10672

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